4MN7
Structural and biochemical analysis of type II free methionine-R-sulfoxide reductase from Thermoplasma acidophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97251 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 120.080, 120.080, 66.911 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.830 - 2.000 |
| R-factor | 0.19467 |
| Rwork | 0.192 |
| R-free | 0.23642 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.859 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.059 | 0.449 |
| Number of reflections | 33454 | |
| <I/σ(I)> | 40.963 | 2.719 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 8.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 60%(v/v) tacsimate , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
