4MN0
Spatial structure of the novel light-sensitive photoprotein berovin from the ctenophore Beroe abyssicola in the Ca2+-loaded apoprotein conformation state
Replaces: 2HPKExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 94 |
| Detector technology | CCD |
| Collection date | 2006-04-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.770, 33.899, 77.406 |
| Unit cell angles | 90.00, 126.74, 90.00 |
Refinement procedure
| Resolution | 40.780 - 1.900 |
| R-factor | 0.19616 |
| Rwork | 0.193 |
| R-free | 0.25554 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.920 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.780 | 2.050 |
| High resolution limit [Å] | 1.900 | 2.000 |
| Completeness [%] | 97.8 | 82.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 298 | 2 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN SOLUTION (15 MG/ML) AND PRECIPITATE SOLUTION (0.2M MAGNESIUM NITRATE HEXAHYDRATE, 20% PEG 3350, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
