4MMM
Human Pdrx5 complex with a ligand BP7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.07244 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.467, 101.228, 58.754 |
| Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
| Resolution | 44.050 - 1.470 |
| R-factor | 0.16383 |
| Rwork | 0.162 |
| R-free | 0.19155 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.380 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.050 |
| High resolution limit [Å] | 1.470 |
| Number of reflections | 119074 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 291 | 22% PEG3350, 0.1M sodium citrate buffer (pH 5.3), 0.2M potassium sodium tartrate, 5mM 1,4-dithio-dl-threitol, 0.02%(w/v) sodium azide, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
