4MMK
Q8A Hfq from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-04-30 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.590, 116.540, 66.620 |
| Unit cell angles | 90.00, 119.98, 90.00 |
Refinement procedure
| Resolution | 33.311 - 2.156 |
| R-factor | 0.2288 |
| Rwork | 0.216 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1s |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.295 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1418)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.250 |
| High resolution limit [Å] | 2.156 | 2.156 |
| Rmerge | 0.089 | 0.322 |
| Number of reflections | 45536 | |
| <I/σ(I)> | 10.68 | 2.64 |
| Completeness [%] | 95.8 | 75.7 |
| Redundancy | 3.96 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 303 | 7% w/v PEG2000 MME, 2% MPD, 20 micromolar zinc chloride, 50 mM Tris-HCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 303.0K |
