4MLZ
Crystal structure of periplasmic binding protein from Jonesia denitrificans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.678, 72.741, 71.306 |
| Unit cell angles | 90.00, 101.32, 90.00 |
Refinement procedure
| Resolution | 35.800 - 1.720 |
| R-factor | 0.138 |
| Rwork | 0.135 |
| R-free | 0.19300 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.301 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.720 | 1.730 |
| Rmerge | 0.107 | 0.581 |
| Number of reflections | 62981 | |
| <I/σ(I)> | 19.9 | 2.09 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 4.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M CaCl2, 0.1M Tris, 25% PEG4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
