4MLW
Crystal structure of non-myristoylated recoverin at 1.45 A resolution with calcium bound to EF-hand 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 4 |
| Unit cell lengths | 84.720, 84.720, 58.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.857 - 1.450 |
| R-factor | 0.1907 |
| Rwork | 0.190 |
| R-free | 0.21220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.160 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.860 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.042 | 0.411 |
| Number of reflections | 36801 | |
| <I/σ(I)> | 17.6 | 3.6 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 1.8 M Ammonium citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
