4ML8
Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.210, 76.670, 201.038 |
| Unit cell angles | 90.00, 92.08, 90.00 |
Refinement procedure
| Resolution | 38.370 - 2.700 |
| R-factor | 0.1895 |
| Rwork | 0.187 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.860 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 76884 | |
| Completeness [%] | 99.6 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 100 mM Nacitrate pH5.6, 20% 2-propanol, 20% PEG 4K, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
