4MKY
Polymerase Domain from Mycobacterium tuberculosis Ligase D in complex with an annealed double-strand DNA break.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-10 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.580, 80.110, 118.390 |
| Unit cell angles | 90.00, 111.62, 90.00 |
Refinement procedure
| Resolution | 37.640 - 2.400 |
| R-factor | 0.19969 |
| Rwork | 0.197 |
| R-free | 0.24529 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iru |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.268 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 110.061 | 46.008 | 2.530 |
| High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
| Rmerge | 0.032 | 0.825 | |
| Number of reflections | 58964 | ||
| <I/σ(I)> | 12.3 | 16.9 | 0.9 |
| Completeness [%] | 98.5 | 99.5 | 96.1 |
| Redundancy | 6.6 | 7.1 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 20% PEG 3350, 200mM Ammonium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
