4MKU
Frog M ferritin mutant H54Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.885 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 184.580, 184.580, 184.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.640 - 1.300 |
| R-factor | 0.12383 |
| Rwork | 0.123 |
| R-free | 0.13851 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ka3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.012 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 21.170 | 21.170 | 1.370 |
| High resolution limit [Å] | 1.300 | 4.110 | 1.300 |
| Rmerge | 0.097 | 0.037 | 0.401 |
| Number of reflections | 66237 | ||
| <I/σ(I)> | 15.2 | 29.3 | 5.9 |
| Completeness [%] | 100.0 | 99 | 100 |
| Redundancy | 14.3 | 13.1 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 1.6-2M magnesium chloride, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
