4MKQ
Crystal structure of the Pore-Forming Toxin Monalysin mutant deleted of the membrane-spanning domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.97887 |
| Spacegroup name | P 64 |
| Unit cell lengths | 129.458, 129.458, 78.888 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.940 - 2.650 |
| R-factor | 0.1888 |
| Rwork | 0.187 |
| R-free | 0.21570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mjt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.132 | 0.534 |
| Number of reflections | 21936 | |
| <I/σ(I)> | 7.8 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M sodium cacodylate, 0.2M calcium acetate, 18% PEG8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
