4MKN
Crystal structure of chloroplastic triosephosphate isomerase from Chlamydomonas reinhardtii at 1.1 A of resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.885 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.814, 93.211, 100.434 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.610 - 1.100 |
| R-factor | 0.16109 |
| Rwork | 0.160 |
| R-free | 0.17717 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r2r |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.672 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.610 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.038 | 0.511 |
| Number of reflections | 111449 | |
| <I/σ(I)> | 19.5 | 3.6 |
| Completeness [%] | 98.0 | 92.7 |
| Redundancy | 6.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 65% (v/v) MPD, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
