4MJG
Crystal structure of a DUF4853 family protein (ACTODO_00621) from Actinomyces odontolyticus ATCC 17982 at 2.65 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91837,0.97941,0.97868 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 97.080, 97.080, 105.044 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.544 - 2.650 |
R-factor | 0.2351 |
Rwork | 0.234 |
R-free | 0.26090 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.023 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | SHELX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.544 | 44.544 | 2.720 |
High resolution limit [Å] | 2.650 | 11.850 | 2.650 |
Rmerge | 0.042 | 0.811 | |
Total number of observations | 570 | 3833 | |
Number of reflections | 15164 | ||
<I/σ(I)> | 9.2 | 10.5 | 0.9 |
Completeness [%] | 88.9 | 86.4 | 95.3 |
Redundancy | 3.2 | 2.8 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 2.4M ammonium sulfate, 0.1M Bicine pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |