4MJC
Synechocystis sp. PCC 6803 glutaredoxin A - P84R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2013-01-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1270 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.230, 38.820, 50.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.870 - 1.409 |
| R-factor | 0.1821 |
| Rwork | 0.178 |
| R-free | 0.21570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qmx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.298 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.067 | 0.319 |
| Number of reflections | 14533 | |
| <I/σ(I)> | 9.6 | 3.2 |
| Completeness [%] | 98.8 | 99.8 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1.5M Ammonium Sulfate, 1% PEG 400, 10mM HEPES, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
