4MJA
Synechocystis sp. PCC 6803 glutaredoxin A-A75I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2013-07-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.2320 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.180, 38.320, 51.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.171 - 2.000 |
R-factor | 0.2081 |
Rwork | 0.206 |
R-free | 0.22920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qmx |
RMSD bond length | 0.030 |
RMSD bond angle | 2.132 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.073 |
Number of reflections | 5603 |
<I/σ(I)> | 16 |
Completeness [%] | 99.9 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1.5M Ammonium Sulfate, 1% PEG400,10mM HEPES, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |