4MIX
PaToxG Glycosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.817, 42.127, 116.367 |
| Unit cell angles | 90.00, 99.52, 90.00 |
Refinement procedure
| Resolution | 56.920 - 1.800 |
| R-factor | 0.2245 |
| Rwork | 0.222 |
| R-free | 0.26297 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.284 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AutoSol (- Phenix) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.380 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.023 | |
| Number of reflections | 64580 | |
| <I/σ(I)> | 1.1 | |
| Completeness [%] | 98.8 | 98.5 |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 20% PEG 3350, 500 mM NaCl, 100 mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
