4MIG
Pyranose 2-oxidase from Phanerochaete chrysosporium, recombinant wild type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.544, 166.448, 91.844 |
| Unit cell angles | 90.00, 106.41, 90.00 |
Refinement procedure
| Resolution | 44.050 - 1.800 |
| R-factor | 0.1736 |
| Rwork | 0.173 |
| R-free | 0.21427 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.003 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.950 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 235360 | |
| <I/σ(I)> | 12.8 | 2.3 |
| Completeness [%] | 99.0 | 98.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M TRIS, 0.1 M MnCl2, 30% (w/v) PEG 400, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
