4MI7

Crystal Structure of Salmonella Effector Protein GtgE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 41 21 2
Unit cell lengths	56.181, 56.181, 125.124
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.630 - 1.650
R-factor	0.1949
Rwork	0.193
R-free	0.22970
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.749
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.650
Number of reflections	23155

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	Lithium Sulfate, Ammonium Sulfate, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K