4MHV
Crystal structure of the PNT domain of human ETS2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 64.440, 64.440, 263.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.950 - 2.450 |
| R-factor | 0.2307 |
| Rwork | 0.230 |
| R-free | 0.23620 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.102 | 0.015 |
| Number of reflections | 12894 | |
| <I/σ(I)> | 17.8 | 2.3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 14.1 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M Cacadylate, pH 6.5, 14 % PEG 10K, 20 % Glycerol, 0.16 M Calcium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
