4MHQ
Crystal structure of human primase catalytic subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2013-01-19 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.28262 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 84.762, 84.762, 147.622 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.060 - 2.200 |
| R-factor | 0.17871 |
| Rwork | 0.176 |
| R-free | 0.23305 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.182 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.076 | 0.976 |
| Number of reflections | 25240 | |
| <I/σ(I)> | 9.5 | 1.506 |
| Completeness [%] | 89.6 | 91.5 |
| Redundancy | 8.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | 0.1M Na-acetate, 0.2M Tri-Na-citrate, 20% PEG3350, 0.1M Na-acetate , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
