4MHI

Crystal structure of a H5N1 influenza virus hemagglutinin from A/goose/Guangdong/1/96

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Detector technology	CCD
Collection date	2010-01-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 1 21 1
Unit cell lengths	72.482, 225.728, 211.633
Unit cell angles	90.00, 98.97, 90.00

Refinement procedure

Resolution	49.657 - 2.595
R-factor	0.192
Rwork	0.189
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbm
RMSD bond length	0.009
RMSD bond angle	1.310
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.595	2.595
Number of reflections	196526
<I/σ(I)>	22.8	1.9
Completeness [%]	95.3	75.6
Redundancy		3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	295	0.1 M Tris, pH 8.2, 21% MPEG2000, VAPOR DIFFUSION, SITTING DROP, temperature 295K