4MGX

Crystal structure of human filamin C domains 4-5 and GPIB alpha cytoplasmic domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-03
Detector	PSI PILATUS 6M
Wavelength(s)	0.976250
Spacegroup name	P 43 2 2
Unit cell lengths	111.800, 111.800, 59.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.730 - 3.160
R-factor	0.2288
Rwork	0.227
R-free	0.27680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v8o
RMSD bond length	0.012
RMSD bond angle	1.806
Data scaling software	XSCALE
Phasing software	PHASER (2.5.1)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.730	47.730	3.070
High resolution limit [Å]	3.000	13.400	3.000
Rmerge	0.196
Number of reflections	8054	119	569
<I/σ(I)>	8.53	29.95	0.29
Completeness [%]	99.8	96	98.4
Redundancy	9.72

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	0.1 M HEPES, pH 7.5, 20% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 297K