4MGS
BiXyn10A CBM1 APO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Detector technology | CCD |
| Collection date | 2013-07-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 52.737, 90.659, 30.998 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.790 - 1.800 |
| R-factor | 0.1616 |
| Rwork | 0.159 |
| R-free | 0.18560 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.024 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.793 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.501 | |
| Number of reflections | 14363 | |
| <I/σ(I)> | 2.38 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 296 | 2.5 M Ammonium Sulfate, 0.1 M Bicine, pH 9.0, 42 mg/mL, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
