4MFJ
The crystal structure of acyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2011-10-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97893 |
Spacegroup name | P 62 |
Unit cell lengths | 105.500, 105.500, 133.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.900 |
R-factor | 0.18267 |
Rwork | 0.180 |
R-free | 0.22853 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.437 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 17598 | |
Completeness [%] | 98.8 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Tris, 2.5M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |