4MEY
Crystal structure of Escherichia coli RNA polymerase holoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-07 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 186.390, 207.238, 308.302 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.917 - 3.948 |
R-factor | 0.2771 |
Rwork | 0.276 |
R-free | 0.32450 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.884 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((AutoMR)) |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.970 |
High resolution limit [Å] | 3.900 | 3.900 |
Rmerge | 0.115 | 0.658 |
Number of reflections | 103841 | |
<I/σ(I)> | 13.227 | 1.797 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 5.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.1M HEPES, 0.2M calcium chloride, 18% PEG400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |