4MEG
In vitro evolved glmS ribozyme triple mutant, magnesium ion complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 166.900, 40.400, 73.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.880 - 3.100 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z75 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.900 | 0.732 |
| <I/σ(I)> | 13.8 | 3 |
| Completeness [%] | 98.2 | 85 |
| Redundancy | 8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 5% PEG 8000, 0.5M lithium chloride, 25mM magnesium acetate, 25mM magnesium chloride, 0.5mM spermine, 25mM cacodylate, 25mM HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
