4ME3

1.8 Angstrom Crystal Structure of the N-terminal Domain of an Archaeal MCM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9
Spacegroup name	P 61
Unit cell lengths	93.460, 93.460, 56.231
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.980 - 1.794
R-factor	0.1844
Rwork	0.182
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.725
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.830
High resolution limit [Å]	1.794	4.880	1.794
Rmerge	0.072	0.041	0.540
Number of reflections	26333
<I/σ(I)>	13.2
Completeness [%]	100.0	99.9	100
Redundancy	12.4	11.8	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	0.1 M HEPES, pH 7.5, 0.2 M magnesium chloride, 15% PEG400, VAPOR DIFFUSION, temperature 291K
1	VAPOR DIFFUSION	7.5	291	0.1 M HEPES, pH 7.5, 0.2 M magnesium chloride, 15% PEG400, VAPOR DIFFUSION, temperature 291K