4MDY
Crystal structure of periplasmic solute binding protein from Mycobacterium smegmatis str. MC2 155
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97899 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.606, 90.449, 33.974 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.820 - 1.780 |
| R-factor | 0.149 |
| Rwork | 0.146 |
| R-free | 0.20050 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.068 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.091 | 0.992 |
| Number of reflections | 24908 | |
| <I/σ(I)> | 23.1 | 1.92 |
| Completeness [%] | 97.3 | 97.3 |
| Redundancy | 6.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 289 | 0.2M Sodium Citrate, 0.1 M Phosphate-citrate, 20 % PEG 8000, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
