4MDU
Crystal structure of apo-Annexin (Sm)1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-25 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.37764, 0.953759 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.729, 87.838, 68.848 |
| Unit cell angles | 90.00, 111.14, 90.00 |
Refinement procedure
| Resolution | 24.200 - 2.200 |
| R-factor | 0.1924 |
| Rwork | 0.190 |
| R-free | 0.24400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.039 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.200 | |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 38248 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 289 | 2.5% PEG2000, 50 mM TRIS, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
