4MCX
P. vulgaris HIGBA structure, crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 62 |
| Unit cell lengths | 120.503, 120.503, 64.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.790 - 2.100 |
| R-factor | 0.174 |
| Rwork | 0.173 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mct |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.179 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.079 | 0.957 |
| Number of reflections | 30863 | |
| <I/σ(I)> | 17.3 | 1.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 293.15 | 25% PEG 4000, 90 mM sodium acetate pH 4.6, 180 mM ammonium acetate, 4% acetone (w/v), VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
