4MCI
Crystal structure of uridine phosphorylase from vibrio fischeri es114 complexed with DMSO, NYSGRC Target 029520.
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-13 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9791 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 163.993, 163.993, 58.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.340 - 2.010 |
| R-factor | 0.1789 |
| Rwork | 0.178 |
| R-free | 0.19860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lnh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.219 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 81.996 | 81.996 | 2.120 |
| High resolution limit [Å] | 2.010 | 6.360 | 2.010 |
| Rmerge | 0.059 | 0.482 | |
| Total number of observations | 4395 | 10720 | |
| Number of reflections | 19810 | ||
| <I/σ(I)> | 12.2 | 10.3 | 1.6 |
| Completeness [%] | 98.2 | 96.7 | 89.4 |
| Redundancy | 6.7 | 6.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2 M AMMONIUM SULFATE, 0.1 M HEPES:NAOH, PH 8.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
