4MC7
Crystal structure of a subtype N11 neuraminidase-like protein of A/flat-faced bat/Peru/033/2010 (H18N11)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-27 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 123.374, 164.358, 214.912 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.835 - 2.990 |
R-factor | 0.21667 |
Rwork | 0.214 |
R-free | 0.26009 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.422 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0111) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.835 | 3.110 |
High resolution limit [Å] | 2.990 | 2.990 |
Number of reflections | 39587 | |
Completeness [%] | 94.5 | 93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 mM calcium acetate, 10% PEG8000, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |