4MC5

Crystal structure of a subtype H18 hemagglutinin homologue from A/flat-faced bat/Peru/033/2010 (H18N11)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	239.093, 239.093, 161.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.019 - 2.238
R-factor	0.1765
Rwork	0.175
R-free	0.20410
RMSD bond length	0.005
RMSD bond angle	0.966
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.238	2.238
Number of reflections	111074
Completeness [%]	99.8	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	293	0.05 M calcium chloride, 30% PEG550 MME, 0.1 M Bis-Tris, pH 6.5, under oil, MICROBATCH, temperature 293K