4MBR
3.65 Angstrom Crystal Structure of Serine-rich Repeat Protein (Srr2) from Streptococcus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 97.598, 97.598, 173.269 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.620 - 3.650 |
| R-factor | 0.21465 |
| Rwork | 0.213 |
| R-free | 0.24073 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mbo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.724 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.710 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Rmerge | 0.050 | 0.690 |
| Number of reflections | 9911 | |
| <I/σ(I)> | 41 | 3.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.4 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 295 | Protein: 7.5mg/mL, 0.25M Sodium cloride, 0.01M Tris-HCl, pH 8.3. Screen: Trap96 (A11), 5M Sodium cloride. Cryo: 4M Sodium formate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
