4MBP

MALTODEXTRIN BINDING PROTEIN WITH BOUND MALTETROSE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	287
Detector technology	AREA DETECTOR
Collection date	1989-01
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	C 1 2 1
Unit cell lengths	106.680, 68.380, 58.440
Unit cell angles	90.00, 112.00, 90.00

Refinement procedure

Resolution	10.000 - 1.700
R-factor	0.194 *
Rwork	0.188
Structure solution method	ISOMORPHOUS
Starting model (for MR)	3mbp
RMSD bond length	0.019
RMSD bond angle	0.035
Data reduction software	SDMS (DETECTOR SYSTEM (NIELSEN))
Data scaling software	SDMS (DETECTOR SYSTEM (NIELSEN))
Phasing software	CHAIN
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.890
High resolution limit [Å]	1.760	1.760
Rmerge	0.099	0.135
Number of reflections	31236
<I/σ(I)>	18.026	1.57
Completeness [%]	80.0	44
Redundancy	3.7	1.41

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2	4 *	PROTEIN WAS CRYSTALLIZED FROM 18% PEG 8000, 10 MM CITRATE, PH 6.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MBP	3.5 (mg/ml)
2	1	drop	maltodextrin	1 (mM)
3	1	drop	PEG6000	16 (%(w/v))
4	1	reservoir	PEG6000	22 (%(w/v))