4MAP
Crystal structure of Ara h 8 purified with heating
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.922, 87.113, 42.863 |
| Unit cell angles | 90.00, 96.74, 90.00 |
Refinement procedure
| Resolution | 30.460 - 1.900 |
| R-factor | 0.20001 |
| Rwork | 0.197 |
| R-free | 0.25226 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4m9b |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.891 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.048 | 0.360 |
| Number of reflections | 23735 | |
| <I/σ(I)> | 29.4 | 2.75 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M Tris (8.0), 0.2 M Lithium sulfate, 34% (w/v) PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
