4MAK
Crystal structure of a putative ssRNA endonuclease Cas2, CRISPR adaptation protein from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.048, 66.009, 69.972 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.030 - 1.100 |
| R-factor | 0.13629 |
| Rwork | 0.132 |
| R-free | 0.17333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.763 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.030 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.083 | 0.430 |
| Number of reflections | 53336 | |
| <I/σ(I)> | 28 | |
| Completeness [%] | 93.0 | 52 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 20% PEG3350, 0.1M KCl, 10mM MgCl2, Na Citr 0.1M ph 6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
