4MAE
Methanol dehydrogenase from Methylacidiphilum fumariolicum SolV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9718 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.700, 75.500, 136.400 |
| Unit cell angles | 90.00, 97.60, 90.00 |
Refinement procedure
| Resolution | 45.830 - 1.600 |
| R-factor | 0.16263 |
| Rwork | 0.161 |
| R-free | 0.18895 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.198 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.078 | 0.471 |
| Number of reflections | 125838 | |
| <I/σ(I)> | 12.1 | 1.9 |
| Completeness [%] | 89.7 | 55.8 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | RESERVOIR: 22%w/v PEG 8000, 0.2M NaCl, without buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
