4MAC
Crystal structure of CIDE-N domain of FSP27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-07-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 32 |
| Unit cell lengths | 63.354, 63.354, 37.606 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.22478 |
| Rwork | 0.221 |
| R-free | 0.29271 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.972 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.680 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 57364 | |
| Completeness [%] | 40.0 | 94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 28% PEG1500, MMT buffer/NaOH, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
