4MA3
Crystal structure of anti-hinge rabbit antibody C2095
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.350, 79.440, 65.410 |
| Unit cell angles | 90.00, 104.04, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.18063 |
| Rwork | 0.180 |
| R-free | 0.21132 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3QOS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.088 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.053 | 0.327 |
| Number of reflections | 64342 | |
| <I/σ(I)> | 16.1 | 4.1 |
| Completeness [%] | 97.5 | 85.3 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES, pH 6.5, 23% PEG3350, 0.2 M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
