4M9Q
Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.050, 76.830, 172.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 2.500 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23536 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ksh |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.758 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.830 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 22891 | |
| <I/σ(I)> | 30.03 | 5.78 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 14.4 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.2 M ammonium sulfate, 0.1 M MES, pH 6.5, 30% PEG5000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
