4M9O
Crystal Structure of monomeric zebrafish beta-2-microglobulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-03-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.084, 50.926, 54.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.140 |
| R-factor | 0.17486 |
| Rwork | 0.172 |
| R-free | 0.23085 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gbl |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.074 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.170 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.056 | 0.216 |
| Number of reflections | 6117 | |
| <I/σ(I)> | 27.5 | 6.9 |
| Completeness [%] | 98.9 | 99 |
| Redundancy | 5.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 30% w/v polyethylene glycol 8,000, 0.2 M sodium acetate trihydrate in 0.1 M sodium cacodylate trihydrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
