4M9N

DNA Polymerase Beta E295K Soaked with dATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	104
Detector technology	IMAGE PLATE
Collection date	2013-01-16
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	54.523, 79.215, 54.685
Unit cell angles	90.00, 106.24, 90.00

Refinement procedure

Resolution	13.923 - 2.275
R-factor	0.1951
Rwork	0.192
R-free	0.22670
Structure solution method	Isomorphous replacement
RMSD bond length	0.002
RMSD bond angle	0.598
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	14.000	14.000	2.360
High resolution limit [Å]	2.275	4.850	2.275
Rmerge	0.053	0.026	0.245
<I/σ(I)>	17.8
Completeness [%]	97.5	99.9	81.9
Redundancy	3.3	3.5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	14% PEG 3350, 300 mM sodium acetate pH 9, 50 mM HEPES pH 7.5, vapor diffusion, hanging drop, temperature 291K