4M9L

DNA Polymerase Beta E295K Soaked with dCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	104
Detector technology	IMAGE PLATE
Collection date	2013-05-14
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	54.362, 80.198, 54.599
Unit cell angles	90.00, 108.59, 90.00

Refinement procedure

Resolution	13.840 - 2.091
R-factor	0.1929
Rwork	0.189
R-free	0.23070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.718
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.1)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	14.000	14.000	2.160
High resolution limit [Å]	2.091	4.460	2.091
Rmerge	0.080	0.035	0.612
<I/σ(I)>	11.9
Completeness [%]	96.5	99.3	91.4
Redundancy	8.8	10.7	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	14% PEG 3350, 300 mM sodium acetate pH 9, 50 mM HEPES pH 7.5, vapor diffusion, hanging drop, temperature 291K