4M9H
DNA Polymerase Beta E295K Soaked with dTTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 104 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-09-27 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.606, 79.460, 54.956 |
| Unit cell angles | 90.00, 105.63, 90.00 |
Refinement procedure
| Resolution | 13.737 - 2.394 |
| R-factor | 0.1822 |
| Rwork | 0.177 |
| R-free | 0.22760 |
| Structure solution method | Isomorphous replacement |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.632 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 14.000 | 14.000 | 2.490 |
| High resolution limit [Å] | 2.394 | 5.090 | 2.400 |
| Rmerge | 0.062 | 0.033 | 0.260 |
| <I/σ(I)> | 15.5 | ||
| Completeness [%] | 99.4 | 99.9 | 95.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 14% PEG 3350, 300 mM sodium acetate pH 9, 50 mM HEPES pH 7.5, vapor diffusion, hanging drop, temperature 291K |
