4M9B
Crystal structure of Apo Ara h 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-22 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | .98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.939, 87.965, 42.898 |
| Unit cell angles | 90.00, 96.78, 90.00 |
Refinement procedure
| Resolution | 29.520 - 1.600 |
| R-factor | 0.18244 |
| Rwork | 0.180 |
| R-free | 0.22221 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ifv |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.126 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.045 | 0.691 |
| Number of reflections | 39702 | |
| <I/σ(I)> | 38.1 | 2.3 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M Magnesium Formate, 20% (w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
