4M9A
Crystal structure of Acyl-coA dehydrogenase from Burkholderia thailandensis E264
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-02 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.260, 111.000, 170.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.870 - 2.200 |
| R-factor | 0.18855 |
| Rwork | 0.186 |
| R-free | 0.23162 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ukw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.540 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.096 | 0.456 |
| Number of reflections | 83921 | |
| <I/σ(I)> | 13.6 | 3.6 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Morpheus a10: Buffer 3 pH 8.5, 0.06M divalents, 30% EDO_Peg8K, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
