4M95

d(ATCCGTTATAACGGAT)complexed with Moloney Murine Leukemia virus reverse transcriptase catalytic fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	108
Detector technology	CCD
Collection date	2013-02-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97903
Spacegroup name	P 21 21 2
Unit cell lengths	54.329, 146.107, 46.972
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.700 - 1.720
R-factor	0.212
Rwork	0.211
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.040
RMSD bond angle	0.954
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	26.700
High resolution limit [Å]	1.720
Rmerge	0.049
Number of reflections	40094
<I/σ(I)>	24.4
Completeness [%]	98.2
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	9% PEG 4000, 5mM Magnesium acetate, 50mM N-(2-acetamido)iminodiacetic acid, pH 6.5, vapor diffusion, hanging drop, temperature 293.15K