4M8M
Crystal structure of the active dimer of zebrafish PlexinC1 cytoplasmic region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.216, 146.100, 209.581 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.164 - 3.307 |
| R-factor | 0.2283 |
| Rwork | 0.226 |
| R-free | 0.28150 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.851 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 24065 | |
| <I/σ(I)> | 19.7 | 1.4 |
| Completeness [%] | 95.8 | 79.5 |
| Redundancy | 10.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.1 | 293 | 0.1M Bis-Tris propane, pH 9.1, 21% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
