4M8A
Crystal Structure of Thermotoga maritima FtsH Periplasmic Domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-04-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924, 0.97901, 0.97134, 0.98696 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 41.809, 65.837, 71.834 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.677 - 1.502 |
| R-factor | 0.1649 |
| Rwork | 0.161 |
| R-free | 0.20180 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.028 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.700 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.253 |
| Number of reflections | 32191 | |
| <I/σ(I)> | 27.9 | 13 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 11.5 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 294 | 25% PEG 3350, 0.1M Na citrate (pH 5.5), 0.2M Li2SO4 , VAPOR DIFFUSION, temperature 294K |
