4M88
Crystal structure of extracellular ligand-binding receptor from Verminephrobacter eiseniae ef01-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-21 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.076, 95.810, 50.300 |
| Unit cell angles | 90.00, 111.56, 90.00 |
Refinement procedure
| Resolution | 31.666 - 1.762 |
| R-factor | 0.1738 |
| Rwork | 0.172 |
| R-free | 0.20880 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.103 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 30220 | |
| <I/σ(I)> | 12.8 | 2.43 |
| Completeness [%] | 99.3 | 98.4 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 0.2 M Lithium Sulfate, 0.1 M Sodium Acetate:Acetic Acid pH 4.5, 30% (w/v) PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
